Fig. 5: Loss of ENDOG repressed the starvation-induced DNA damage and autophagy. | Nature Communications

Fig. 5: Loss of ENDOG repressed the starvation-induced DNA damage and autophagy.

From: Endonuclease G promotes autophagy by suppressing mTOR signaling and activating the DNA damage response

Fig. 5

a–c Representative images of comet assay in wild-type or ENDOG knockout L02 cells (a) and the quantification of tail DNA (b) and tail moment (c) (starvation treated for 1 or 3 h; n = 75–100 independent cells; data are presented as mean values ± SD, **p < 0.01, ***p < 0.001). d, e Representative images of p-H2A.X foci (d) and quantitative results (e) in wild-type or ENDOG knockout (KO) L02 cells at the indicated time point after the starvation treatment (scale bar = 10 μm, n = 50 independent cells; data are presented as mean values ± SD; **p < 0.01, ***p < 0.001). f–i Western blots and quantitative results of the indicated proteins in wild type or ENDOG knockout L02 cells following starvation treatment (n = 4 independent samples; data are presented as mean values ± SD, *p < 0.05; **p < 0.01; ***p < 0.001). Source data are provided as a Source data file.

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