Table 1 Measured Kd values (in nM) of representative natural and artificial K120R-bound and K120R-unbound REs for WT and K120R-p53.

From: Distinct mechanisms control genome recognition by p53 at its target genes linked to different cell fates

RE name

RE sequence

Kd [nM]

WT

K120R

200 mM NaCl

275 mM NaCl

200 mM NaCl

275 mM NaCl

p21

GAACATGTCCcAACATGTTg

26

23

35

43

MDM2

AGACAAGTCaGGACTTaaCT

20

97

36

72

APBB2

AGGCATGTCCcAACATGCCC

10

40

37

80

3Q05 pdb

GGGCATGTCTGGGCATGTCT

6

33

33

78

BAX

GGGCAgGCCCGGGCTTGTCg

22

460

280

N/A

NOXA

GAGCgTGTCCGGGCAgGTCg

45

446

68

∼2900

“Pro-apoptotic”

GGGCATGCCCGGGCATGCCC

15

254

23

∼3500

6FJ5 pdb

AGGCATGCCTAGGCATGCCT

8

783

140

800

BAX_8/13

GGGCAgGTCCGGACTTGTCg

8

40

94

168

NOXA_3/13

GAACgTGTCCGGACAgGTCg

23

60

14

181

p21_8/13

GAACATGCCCcAGCATGTTg

33

99

55

479

BAX_8/13 I/C

GGGCAgG(I-)CCCGGI(-C)CTTGTCg

9

83

150

N/A

6FJ5_8/18 I/C

AGGCATG(I-)CCTAGGCATG(I-)CCT

8

58

72

691

  1. Lower-case letters in RE sequences indicate mismatches from p53 RE consensus. I (inosine) and C (cytosine) in bold indicate I/C bps. Letters in parenthesis indicate a nucleotide of the I-C bp found on the complementary strand. Underlined are core (CWWG) sequences.
  2. N/A not available.
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