Fig. 1: KSHV infection results in recruitment of DDR and recombination factors to telomeres.

a Analysis of telomere-associated proteome in response to infection. Data is presented as fold-difference in number of unique peptides relative to uninfected co-culture as determined by spectral peak calling. Peptides in scrambled pulldown were subtracted prior to comparison. b Occurrence of APBs in infected BJAB cells. Representative micrograph of the infected cell line and uninfected co-culture is shown. Arrows denote incidences of APBs in merged image, dotted line indicates outline of DAPI counterstain (not shown). c Quantification of cells with APBs. Experiment was performed in biological duplicate on the at least 50 cells from each cell line. Statistical significance was inferred by unpaired, two-tailed t-test (*p = 0.0243). d Quantification of mean telomere signal intensity. Telomere FISH signal intensity of cells included in analysis shown in c were quantified and plotted as dot plot. Significance was tested with an unpaired, two-tailed t-test (****p < 0.0001). e Quantification of signal obtained from C-circle analysis. Experiment was performed in technical quadruplicate and quantification was carried out by subtracting the Φ29-negative controls. Dotted line represents threshold, which determined positive samples (>5). Statistical significance was tested by unpaired, two-tailed Mann–Whitney test (*p = 0.028). Representative blot shown on the right next to quantification. f Representative confocal micrographs of pRPA TIF staining, inset presents digital zoom of area marked by dashed box in main image. Dotted line denotes the outline of DAPI nuclear counterstain (not shown) and arrows show pRPA TIFs which were counted in the analysis. g Quantification of occurrence of pRPA TIFs depicted in f. Experiment was performed in biological quadruplicate with 8 independently established KSHV positive cell lines. Each experiment analysed at least 50 cells per condition. Statistical significance was inferred by Mann–Whitney test (all *p = 0.0286). h, i Analysis of the occurrence of APBs in an analogous manner to pRPA TIF analysis f, g using the same biological quadruplicate, presentation style and statistical test. All error bars represent SEM.