Fig. 6: SPOP-WT is an ERG target gene and required for ERG-mediated cell invasion.
From: Dual functions of SPOP and ERG dictate androgen therapy responses in prostate cancer
a SPOP mRNA expression levels in 333 primary prostate cancer tissues stratified according to the indicated driver mutations3. Error bars, mean ± s.d. b SPOP mRNA and protein levels in response to forced expression of ΔERG in PC3 prostate cancer cells by qPCR and immunoblotting, respectively. Error bars, mean + s.e.m. (n = 3). P-values were determined by unpaired, two-tailed Student’s t-test. Control vs. ΔERG for SPOP expression levels. Control vs. ΔERG for ERG expression levels. c Transwell Matrigel invasion assay of PC3 cells with forced expression of ΔERG and knockdown of SPOP using two different short-hairpin RNAs. Protein expression of the indicated proteins was assessed in parallel by immunoblotting. Error bars, mean ± s.e.m. (n = 3) (bar represents 400 µm). d Transwell Matrigel invasion assay of PC3 cells with forced expression of ΔERG and HA-ZMYND11-DMT2 and corresponding immunoblot analysis. Error bars, mean ± s.e.m. (n = 3) (bar represents 400 µm). e Analysis of the ΔERG- and HA-ZMYND11-DMT2-induced transcriptional changes in the ERG target genes PLAU and PLAT. All error bars, mean ± s.e.m. P-values were determined by one-way ANOVA with multiple comparisons and adjusted using Benjamini–Hochberg post-test (a, c, d, e). NS, not significant. Molecular weights are indicated in kilodaltons (kDa). Source data are provided as a Source Data File.
