Fig. 4: The influx of calcium ions via SOCE support expansion of membrane blebs.
From: STIM-Orai1 signaling regulates fluidity of cytoplasm during membrane blebbing
Membrane blebbing of DLD1 cells expressing either Sec61β-mCherry and GFP-PLCδ-PH (a, Supplementary Movie 5) or Orai1-mCherry and GFP-tagged STIM1 (b). Arrowheads show the ER-PM contact sites. Indicated times are relative to the first image. Results shown are representative of three independent experiments. Scale bar, 2 μm. c–f DLD1 cells expressing Lifeact-RFP were treated with the SOCE inhibitors AnCoA4 (50 µM) and SKF96365 (10 µM). c Representative images from three independent experiments. Indicated times are relative to drug treatment. Scale bar, 10 μm. The number (d, N = 20 cells), area (e, N = 20 blebs) and retraction velocity (f, N = 20 blebs) of membrane blebs in vehicle-treated (control) and drug-treated (4-bromo-A23187) DLD1 cells over 10 min from three independent experiments. Individual data points are plotted with the means ± SD. ****P < 0.0001 (One-way ANOVA with Tukey’s post-hoc multiple comparison test). Source data are provided as a Source Data file.
