Fig. 4: In vitro TNF–TNFR1 network assembly. | Nature Communications

Fig. 4: In vitro TNF–TNFR1 network assembly.

From: Structural insights into the disruption of TNF-TNFR1 signalling by small molecules stabilising a distorted TNF

Fig. 4

a Aggregation of hTNF (3 mg/mL) +hTNFR1(41–201) (orange trace) and hTNF (3 mg/mL) +hTNFR1(41–184) (blue trace) over time (ratio of TNF:TNFR = 1:2.16). b Aggregation of hTNF +hTNFR1(41–201) over a range of concentrations and at two ratios of hTNF:hTNFR1 (1:1.2 blue bars and 1:2.16 red bars). c Aggregation state of hTNF (2 mg/mL) +hTNFR1 at equilibrium over a range of ratios. d Aggregation of hTNF (2 mg/mL) +hTNFR1 at five ratios (1:1.7 blue trace, 1:2 orange trace, 1:2.4 grey trace, 1:3 yellow trace, 1:3.5 green trace) over time. e Aggregation of hTNF (2 mg/mL) + hTNFR1 over a range of ratios with UCB-9260 (10-fold excess over hTNF trimer) (grey bars) and DMSO only (orange bars) (n = 1 independent experiments). f Aggregation of hTNF +hTNFR1 at a fixed ratio of TNF:TNFR = 1:3.2 over a range of total protein concentrations with UCB-9260 (5-fold excess over TNF) (blue trace) (n = 1 independent experiments, a similar rightward shift in the point of aggregation was observed for multiple compounds—Supplementary Fig. 3) and DMSO control (red trace) (n = 2 independent experiments). Source data are provided as a Source Data file.

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