Fig. 5: Effect of adsorbed serum proteins on preosteoblast cell adhesion to DM50CO50-modified surfaces.
From: Dual mechanism β-amino acid polymers promoting cell adhesion
a The amount of surface-adsorbed total protein after variable surfaces were incubated with a serum-containing cell culture medium for 2 h. b, c AFM images of DM50CO50 and RGD-modified surfaces after incubation with serum-containing cell culture medium (b) and pure fibronectin (c) for 2 h. d The amount of surface-adsorbed individual protein (fibronectin, vitronectin, collagen, laminin) on DM50CO50 and RGD-modified surfaces after cells were cultured on the surfaces for 2 days. Fluorescence micrographs of: e live/dead stained cells, f spreading area, and g density analysis, of cells 2 h after seeding on DM50CO50 and RGD-modified surfaces, which were first incubated with serum-containing medium and then treated with or without integrin αvβ3 before cell seeding. h Carton illustration of results in (e–g). Data in (a, d, f, g) represent mean ± s.d. (n = 3 for a, d and n = 9 for f, g). Statistical analysis: two-tailed t-test, **p < 0.001, ***p < 0.0001.
