Fig. 7: Demonstration of cell adhesion impact of DM50CO50-incorporated hydrogel on in vivo bone regeneration using a critical-size rat calvarial defect model. | Nature Communications

Fig. 7: Demonstration of cell adhesion impact of DM50CO50-incorporated hydrogel on in vivo bone regeneration using a critical-size rat calvarial defect model.

From: Dual mechanism β-amino acid polymers promoting cell adhesion

Fig. 7

a Synthesis of bare PEG hydrogel and DM50CO50-modified PEG hydrogel. b Comparison before and after PEG hydrogel swelling. c Preosteoblast cells adhesion to bare PEG hydrogel and DM50CO50-modified PEG hydrogel after cell seeding for 2 days. Cells were subjected to live/dead staining. d Masson’s trichrome stain, e 3D micro-CT analysis, and f bone volume analysis, of the cranial bone samples from bare PEG hydrogels, DM50CO50-modified PEG hydrogels, GelMA, and PLA after 8 weeks of implantation. Data in (f) represent mean ± s.d. (n = 6). Statistical analysis: two-tailed t-test, *p < 0.05.

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