Table 1 Values of k1, k-1, kinact, kreact (the latter at −0.196 V, all at 5 °C) for CbA5HWT and variants.

From: A safety cap protects hydrogenase from oxygen attack

 

k1 (s−1)

k−1 (s−1)

kinact (s−1)

kreact (s−1)

WT (pH 10)

0.095

0.019

0.65

0.015

WT (pH 8.5)

0.068

0.018

0.54

0.069

WT (pH 7)

0.061

0.016

0.42

0.17

A561F (pH7)

0.025

0.058

0.28

0.19

L364F (pH7)

0.055

0.017

0.14

1.45

P386L (pH7)

0.049

0.051

0.15

1.50

A561F-L364F (pH 7)

0.017

0.067

0.13

2.09

Accuracy

8%

28%

7%

6%

  1. Rate constants were obtained by analyzing kinetic data of anaerobic inactivation such as those shown in Fig. 4a with the “AAI” model (Supplementary Fig. 14 shows the dependence of kreact on E). The CbA5HWT reference for the kinetic parameters of the variant proteins, measured at pH7 is marked in bold. The % accuracy is our estimate of the maximal error for the determination of each rate constant.
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