Fig. 2: Diverse murine macrophage populations are sensitive to polymersome surface chemistry in vivo.
a Overview of nanocarrier biodistribution study in four treatment groups of C57BL/6 J mice (n = 5) receiving intravenously administered PBS or near infrared dye, DiR-loaded PS (DiR PS). After 4 h, organ and cellular biodistribution was assessed by IVIS and multicolor flow cytometry, respectively. b Percent change in DiR PS fluorescence (λEx = 750 nm; λEm = 780 nm) between serum (4 h) and a formulation-specific t0 proxy. The t0 proxy is the input DiR PS formulation diluted in untreated mouse plasma in a 1:15 ratio matching the ratio of DiR PS (100 μL) administered to the ~1.5 mL blood volume per mouse. c–h Organ biodistribution. c Representative IVIS images of organs. The radiant efficiency is displayed. d Distribution of total adjusted radiant efficiency within each treatment group, representing the percentage of the total adjusted radiant efficiency accounted for by the spleen, liver, kidneys, and lungs. e–h Adjusted radiant efficiency comparison between treatment groups in the e spleen, f liver, g kidneys, and h lungs. i–m Cellular biodistribution. The percentage of DiR PS+ cells of the specified type is displayed for the i spleen, j liver, k lymph nodes (LNs), l lungs, and m kidneys. Statistical significance was determined by ANOVA with post hoc Tukey’s multiple comparisons test (5% significance level; *p < 0.05, **p < 0.01, ***p < 0.0005, ****p < 0.0001). Error bars represent s.e.m. n Percentage of the 31 statistically significant comparisons (annotated in i–m) accounted for by each cell type.
