Fig. 6: Epithelial expression levels of Gata4 and Sox2 regulate the regionally restricted activation of MAPK/ERK via Fgfr10/Fgfr2 axis. | Nature Communications

Fig. 6: Epithelial expression levels of Gata4 and Sox2 regulate the regionally restricted activation of MAPK/ERK via Fgfr10/Fgfr2 axis.

From: Epithelial expression of Gata4 and Sox2 regulates specification of the squamous–columnar junction via MAPK/ERK signaling in mice

Fig. 6

a RNA-ISH analyses of Fgf10, Fgfr2, and Gata4 for wild-type stomachs at E11.5, E13.5, and E15.5. Arrows indicate the Fgfr2-expressing proximal epithelial cells. Arrowheads indicate the Fgf10-expressing distal mesenchymal cells. n = 4. Scale bar, 100 µm. b IF analyses of pERK, P63, and GATA4 for the stomachs of the Gata4 cKO (E11.5) embryos at E13.5. Dash lines demarcated the Gata4-depleted cells. n = 3. Scale bar, 100 µm. c IF analyses of pERK and KRT7 for the stomach of the Gata4 cKO (E11.5) embryo at E18.5. Arrows indicate the pERK+KRT7+ transitional epithelial cells in the distal stomach. n = 3. Scale bar, 100 µm. d Left: a scheme for the Gata4 KO experiments using Gata4CreERT2/flox mice. Right: IHC analyses of GATA4 for the stomachs of the Gata4CreERT2/flox embryos at E13.5. n = 3. Scale bar, 100 µm. e RNA-ISH analyses of Fgf10 and Gata4 for the stomachs of the Gata4CreERT2/flox embryos at E13.5. n = 3. Scale bar, 100 µm. f Left: a scheme for the Sox2 cKO experiment using Sox2CreERT2/flox mice. Right: IHC analyses of SOX2 for the stomachs of the Sox2CreERT2/flox embryos at E13.5. n = 3. Scale bar, 100 µm. g Left: RNA-ISH analyses of Fgfr2 and Gata4 for the Sox2 cKO (E11.5) stomachs at E13.5. n = 3. Scale bar, 100 µm. Right: Q-PCR analyses of Sox2, p63, and Fgfr2 for the epithelial cells of control and Sox2 cKO (E11.5) stomachs at E13.5. The CT value of each gene is normalized by B2M. The average ΔCT values of control stomachs are set to 1. n = 3 independent experiments. Data are presented as mean values ± SD, two-sided t-test. h Left: IHC analyses of pERK for the stomachs of wild type, Gata4CreERT2/flox, and Sox2CreERT2/flox embryos at E13.5. Arrows indicate the pERK+ cells in the stomach epithelium. Scale bar, 100 µm. Right: quantification of pERK+ cells in the proximal stomach epithelium. n = 3 independent experiments. Data are presented as mean values ± SD, one-way ANOVA. i A scheme illustrates that epithelial Gata4 and Sox2 regulate the axial patterning of Fgf10/Fgfr2 and regionally restricted activation of MAPK/ERK.

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