Fig. 5: Main classes of de novo transcripts in yeast.
From: Uncovering de novo gene birth in yeast using deep transcriptomics
a Diagram of a pair of overlapping genes, which are on opposite strands. We consider all pairs of transcripts with any antisense overlap (no minimum overlap threshold). b Fraction of transcripts in each conservation level which overlap genes on the opposite strand. A higher proportion of de novo transcripts are in this orientation relative to more conserved transcripts. c Correlation fold change (FC) expression values overlapping genes. The differential expression (normal vs. oxidative stress) of gene pairs, which are overlapping each other on opposite strands is strongly correlated (R = 0.73 Spearman’s correlation, p-value < 10−5) suggesting they may be co-regulated. d Diagram of a divergent pair of gene. The genes are in a head-to-head orientation on opposite strands and can share a single bidirectional promoter. We considered transcripts separated by 1-400nt to be divergent. e Fraction of transcripts in each conservation level, which are in a divergent orientation. f Correlation FC expression values divergent genes. The differential expression (normal vs. oxidative stress) of divergent gene pairs is only weakly correlated (R = 0.28 Spearman’s correlation, p-value = 0.02754). Source data are provided as a Source Data file.
