Fig. 6: HEI3090 combined with αPD-1 induces antitumor memory immune response.

a Schematic illustration of treatments with transfer of CD8 cells. b Average tumor area of LLC allograft in 90-day-old WT and 90-day-old cured mice in absence of treatment. Curves showed mean tumor area in mm² ± SEM (n = 7 per group, two-way Anova test). c Survival curves of animals from the study shown in b. Curves showed survival (n = 7 per group, Mantel Cox test). d Average tumor area of LLC allograft in WT mice injected with CD8⁺ T cells isolated from rechallenged cured mice as shown in a. Curves showed mean tumor area in mm² ± SEM (n = 4 per group, two-way Anova test). e Long-lasting antitumor immune response: schematic illustration of treatments. f Mouse body weight follow up of 300-day-old cured mice injected with anti-isotype (black circle) or depleting αCD8 antibodies (blue circle). Each curve represents one mouse (n = 6 per group). g Individual survival curves of 340-day-old WT and cured animals injected with anti-CD8 antibody and rechallenge with LLC in absence of treatment. (n = 6 per group). h Average tumor area from animals shown in g. Data are presented by violin plots showing all points with hatched bar corresponding to median of tumor area (n = 6 per group. Two-tailed Mann–Whitney test). i Survival curves from animals shown in g. (n = 6 per group, Mantel Cox test). p values: *p < 0.05, **p < 0.01 ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.