Fig. 1: TAPSβ for bisulfite-free 5mC-specific sequencing. | Nature Communications

Fig. 1: TAPSβ for bisulfite-free 5mC-specific sequencing.

From: Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution

Fig. 1

a Schematic demonstration of TAPSβ. b Conversion rates of TAPSβ at known 5mCG or 5hmCG positions from CpG-methylated lambda DNA or synthetic spike-in. c False-positive rate of TAPSβ from 2-kb-unmodified spike-in. d Correlation analysis between TAPSβ and published RRoxBS-seq dataset at CpGs with the minimal depth of 10. The color scale represents density. e Correlation analysis between TAPSβ and published oxBS-seq dataset at CpGs with the minimal depth of 10. The Pearson’s r is shown at the top. The raw signal for each CpG was calculated as the ratio between C and the sum of C and T.

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