Fig. 3: Comparison of CAPS with other methods. | Nature Communications

Fig. 3: Comparison of CAPS with other methods.

From: Subtraction-free and bisulfite-free specific sequencing of 5-methylcytosine and its oxidized derivatives at base resolution

Fig. 3

a Comparison of 5mC and 5hmC levels tiled by 1-kb bins for TAPSβ, CAPS, ACE-seq, and TAB-seq. The levels of unmodified and modified cytosines were estimated by MLML using the direct readout from the method combination shown at the title of each subfigure. b Example of genome browser view on chromosome 4 showing CAPS-detected consistent 5hmC sites when compared with ACE-seq and TAB-seq. c Pie chart shows the overlap of called 5hmCGs with putative genomic regulatory elements. d The relative enrichment of 5hmCG (blue) and random sites (white) at genomic regulatory elements. ‘Random’ consists of ten random samplings. The mean is shown as the bar height and the error bars denote standard deviation (n = 10 random sampling events). Each dot denotes one random sampling event. The ratios between observed and random are shown at the top.

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