Fig. 5: Catalytic activity of targeted CYP71D subfamily members with putative tanshinone intermediates accumulating in CYP71Ds-RNAi plant roots. | Nature Communications

Fig. 5: Catalytic activity of targeted CYP71D subfamily members with putative tanshinone intermediates accumulating in CYP71Ds-RNAi plant roots.

From: Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza

Fig. 5

Extracted ion chromatograms showing the in vitro catalytic activity of (a) CYP71D375 with 4. b CYP71D373 and CYP71D375 with 5. c CYP71D375 with 6. d CYP71D411 with 3. e CYP71D375 with 8. In each case, enzyme-mediated activity is indicated by the green chromatograms (with the relevant enzyme noted), while those for the empty vector (EV) negative control assays are in black.

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