Fig. 2: Software for selection of NeissLock complexes.
From: NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins
a Considerations for NeissLock complex selection. (i) The target protein (green) should have an α- or ε-amine proximal to the C-terminus (Ct) of the binder protein (blue). (ii) To avoid self-reaction, the binder protein should not feature an amine close to its own C-terminus. (iii) A bulky region on the target protein may sterically impede amine reactivity. (iv) SPM should not occlude the interface between binder and target proteins. b NeissDist database pipeline. c Minimal distances per category from NeissDist. Only C-terminus (Ct) to lysine with a primary distance from C (main-chain carbonyl carbon) to Nε was considered (see methods for N-terminal lysines). Count of those distances below distance cut-off (Å) for all types. d Comparison of those primary distances from NeissDist to identify shortest distance per structure. Count of these distances below distance cut-off (Å) according to type.
