Fig. 4: Stomatal and photosynthetic properties of OSA1-ox plants. | Nature Communications

Fig. 4: Stomatal and photosynthetic properties of OSA1-ox plants.

From: Plasma membrane H+-ATPase overexpression increases rice yield via simultaneous enhancement of nutrient uptake and photosynthesis

Fig. 4

a Representative stomata in the epidermis of WT plants. Experiments were repeated three occasions with similar results. b Percentage of open stomata observed after 3 h of darkness (DK), red light plus blue light (RL + BL) or RL + BL in the presence of 20 μΜ abscisic acid (ABA) in WT and OSA1-ox plants (for the details see the “Methods” section). c, d Stomatal conductance (c) and CO2 assimilation rate under (d) DK (30 min), white light (WL; 2 h) and a second DK treatment (30 min) in WT and OSA1-ox plants. e, f Stomatal conductance (e) and CO2 assimilation rate (f) in response to light in WT and OSA1-ox plants. g Relationship between CO2 assimilation rate and intercellular CO2 concentration in WT and OSA1-ox plants. Small circles in b–d represent data points for individual experiments; three biological replicates were analysed for each treatment. Values in b–d are presented as the means ± SEs (n = 3) and those in e–g are the means ± SDs (n = 3). Differences were evaluated using the two-tailed Student’s t test (*P < 0.05; **P < 0.01). The exact P values are provided in the Source Data file.

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