Fig. 2: Combination of 2 spike protein variants and isotype profiling by multiplex assay increases accuracy to identify SARS-CoV-2 antibody-positive individuals. | Nature Communications

Fig. 2: Combination of 2 spike protein variants and isotype profiling by multiplex assay increases accuracy to identify SARS-CoV-2 antibody-positive individuals.

From: Exploring beyond clinical routine SARS-CoV-2 serology using MultiCoV-Ab to evaluate endemic coronavirus cross-reactivity

Fig. 2

a, b Scatterplot detailing MultiCoV-Ab cut-offs. Signal to cut-off (S/CO) values are displayed for Spike Trimer against RBD on a logarithmic scale. For IgG (a), cut-offs are visualized by straight lines and SARS-CoV-2-infected and uninfected samples are separated by color (black circles – SARS-CoV-2-uninfected; red circles – SARS-CoV-2-infected). For IgA (b) cut-offs are visualized as dashed lines and S/CO of 2 used for the combined cut-off is shown as straight lines. SARS-CoV-2-infected samples are split into IgG-positives and -negatives by color as indicated in the plot. c, d Scatterplots display IgG response to additional SARS-CoV-2 antigens contained in the MultiCoV-Ab panel: MFI for spike subdomains S1 vs S2 (c) or nucleocapsid antigens N vs N-NTD (d) are displayed on a logarithmic scale. SARS-CoV-2-uninfected samples are distinguished from SARS-CoV-2-infected and MultiCoV-Ab classification into positives or negatives as indicated by color. Source data are provided as a Source Data file.

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