Fig. 3: Multiplex-based seroprofiling allows in-depth characterization of SARS-CoV-2 antibody responses. | Nature Communications

Fig. 3: Multiplex-based seroprofiling allows in-depth characterization of SARS-CoV-2 antibody responses.

From: Exploring beyond clinical routine SARS-CoV-2 serology using MultiCoV-Ab to evaluate endemic coronavirus cross-reactivity

Fig. 3

a Kinetic of SARS-CoV-2 antigen-specific IgA and IgG responses is shown for indicated days after symptom onset for six SARS-CoV-2-specific antigens for five different patients. Patients are indicated by color. b, c Samples of SARS-CoV-2-infected individuals were analyzed to identify antigen- and isotype-specific antibody responses based on hospitalization indicating disease severity (b) or age (c). Data is presented as Box-Whisker plots of sample MFI on a logarithmic scale. Box represents the median and the 25th and 75th percentiles, whiskers show the largest and smallest values. Outliers determined by 1.5 times IQR of log-transformed data are depicted as circles. p-value (Mann–Whitney U test, two-sided) is displayed at the top of the boxes, indicating differences between signal distribution for respective groups. Cut-off values for MultiCoV-Ab classification are displayed as horizontal lines (Spike Trimer IgG: 3,000 MFI, IgA: 400 MFI; RBD IgG: 450 MFI, IgA: 250 MFI). Source data are provided as a Source Data file.

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