Fig. 3: LPA signaling initiates cilia disassembly through Aurora A.

a Scatter plot analysis of transcriptome expression profiles of siCtrl LPA 18 h versus siCtrl LPA 0 h, and siLPAR1 LPA 18 h versus siCtrl LPA 18 h samples in RPE-1 cells. Red dots and green dots highlight the significantly upregulated or downregulated expressed genes, respectively. b The Venn diagram shows the overlap of upregulated and downregulated genes in a. c Statistics of enriched GO terms display 251 overlapped genes in (b, the brown part). The size of the point indicates the number of differentially expressed genes in this pathway, and the color of the points corresponds to a different p-value range. d Histogram of the enriched of KEGG pathway of 251 overlapped genes in (b, the brown part). e Heatmaps showing TOP20 enriched genes in (b, the brown part). f Immunoblot analysis was carried out using indicated antibodies. RPE-1 cells were starved for 12 h and then transfected with control siRNA or LPAR1 siRNA. Following serum starvation for another 48 h, cells were treated with 10% FBS or 2 μM LPA for indicated time points. g LPA activates Aurora A through phosphorylation in RPE-1. Ciliated RPE-1 cells were pretreated with Ki16425 (40 μM) or DMSO control for 30 min, and then cells were stimulated with 10% FBS or 2 μM LPA for 2 h. Cells were stained with anti-p-AurA (Aurora A, green) and anti-Ac-tub (Ac-tubulin, red) antibodies or anti-AurA (Aurora A, green) and anti-Ac-tub (Ac-tubulin, red) antibodies, respectively. Scale bar: 5 μm (main image) and 1 μm (magnified region). h The effect of serum- or LPA-induced cilia disassembly in Aurora A knockdown cells. Source data are provided as a Source Data file. Three experiments were repeated independently with similar results in f and g. Data are presented as mean ± S.D. of three independent experiments in h. n, number of cells. ***P < 0.001. Two way ANOVA test was performed followed by Dunnett’s multiple comparisons in h.