Fig. 4: LPA signaling modulates Aurora A through YAP/TAZ and calcium/CaM pathway.

a YAP/TAZ is required for the LPA-induced the transcription of Aurora A. RPE-1 cells were starved for 12 h and then transfected with control siRNA or YAP/TAZ siRNAs. Following serum starvation for another 48 h, cells were treated with 2 μM LPA for 18 h, and the mRNA levels were measured by qPCR. b Immunoblot analysis in control or YAP/TAZ knockdown cells using indicating antibodies. RPE-1 cells were transfected and treated as described in Fig. 3f. c The effect of serum- or LPA-induced cilia disassembly in control or YAP/TAZ knockdown cells. RPE-1 cells were transfected and treated as described in Fig. 2b, c. d CMZ or EGTA blocks serum- and LPA- induced Aurora A activation. Ciliated RPE-1 cells were pretreated with CMZ (5 μM), EGTA (0.5 mM) or DMSO control for 30 min, and then cells were stimulated with 10% FBS or 2 μM LPA for 2 h. Cells were stained with anti-p-AurA (Aurora A, green), anti-Ac-tub (Ac-tubulin, red) antibodies. Scale bar: 5 μm (main image) and 1 μm (magnified region). e CMZ or EGTA blocks serum- and LPA- induced cilia disassembly. Ciliated RPE-1 cells were pretreated with Ki16425 (40 μM), CMZ (5 μM), EGTA (0.5 mM) or DMSO control for 30 min, and then cells were stimulated with 10% FBS or 2 μM LPA for 2 h. f A proposed model for LPA signaling in the regulation of cilia disassembly. Source data are provided as a Source Data file. Three experiments were repeated independently with similar results in b and d. Data are presented as mean ± S.D. of three independent experiments in a, c, and e. n, number of cells. ***P < 0.001. Two-tailed Student’s t-test in a, two-way ANOVA test was performed followed by Dunnett’s multiple comparisons in c and e.