Fig. 1: Interaction of SAMHD1 with short phosphorothioated oligonucleotides. | Nature Communications

Fig. 1: Interaction of SAMHD1 with short phosphorothioated oligonucleotides.

From: Nucleic acid binding by SAMHD1 contributes to the antiretroviral activity and is enhanced by the GpsN modification

Fig. 1

a, b Fluorescence anisotropy (FA)-binding assays reveal that binding of short phosphorothioated oligonucleotides to SAMHD1 is remarkably tolerant to increasing salt concentration compared to phosphodiester-linked (6FAM-ssDNA57 and 6FAM-ssRNA40) oligonucleotide binding47. Location of phosphorothioate bonds is denoted with asterisks in all figures (n = 2 independent experiments). c Oligomerization-defective mutant Y146S/Y154S is impaired in oligonucleotide binding. d Analysis of SAMHD1 ligand-induced oligomerization by analytical ultracentrifugation (AUC) using 280-nm absorbance readout. e AUC analysis using 6FAM fluorescence detection reveals association of oligonucleotides with SAMHD1 dimers and tetramers. Error bars represent s.d. of two replicate measurements performed on distinct samples. Source data are provided as a Source Data File.

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