Fig. 6: Functional effects of H376A, R352A, and K523A mutations. | Nature Communications

Fig. 6: Functional effects of H376A, R352A, and K523A mutations.

From: Nucleic acid binding by SAMHD1 contributes to the antiretroviral activity and is enhanced by the GpsN modification

Fig. 6

a Oligonucleotide-binding affinities of allosteric site mutants measured using the fluorescence anisotropy-binding assay (n = 2 independent experiments). b In vitro dNTPase activities of allosteric site mutants (n = 2 independent experiments). c Oligonucleotide binding and oligomerization properties of allosteric site mutants as monitored by SEC. d Expression of SAMHD1 variants in U937 cells. e dNTP depletion in U937 cells by WT and mutant SAMHD1 variants (n = 2 independent experiments). f HIV-1 restriction activity of WT and mutant SAMHD1 variants (n = 2 independent experiments). Error bars represent s.d. of two replicate measurements performed on distinct samples. Source data are provided as a Source Data File.

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