Fig. 1: The Cavin1 N-terminal and C-terminal DR domains are important for self-association into oligomers. | Nature Communications

Fig. 1: The Cavin1 N-terminal and C-terminal DR domains are important for self-association into oligomers.

From: Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation

Fig. 1

a Schematic representation of Cavin1 and truncations. DR disordered region, HR helical region. b The diffusion rate of Cavin1, Cavin1-ΔDR1 and Cavin1-ΔDR3 in solution assessed by fluorescence correlation spectroscopy (FCS). Bacterially expressed and purified ubiquitin and GFP tagged proteins (Supplementary Fig. 2) were analysed in high NaCl concentration (500 mM) and physiological NaCl concentration (150 mM). Error bars indicate mean ± SD (standard deviation), n = 39 over two independent experiments, ns—not significant, *P < 0.05, ***P < 0.001. c The diffusion rate of GFP-tagged Cavin1, Cavin1-ΔDR1 and Cavin1-ΔDR3 in lysates after expression in MCF7 cells (lacking endogenous Cavins and Caveolins). Buffer contained 150 mM NaCl. Error bars indicate mean ± SD (standard deviation), n = 235 over three independent experiments, ns—not significant, ***P < 0.001. Error bars indicate mean ± SD. Source data for Fig. 1b, c are provided as a Source Data file.

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