Fig. 5: Removing the Cavin1 DR domains prevents deformation of GMV membranes. | Nature Communications

Fig. 5: Removing the Cavin1 DR domains prevents deformation of GMV membranes.

From: Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation

Fig. 5

a Purified Ub-tagged and GFP-tagged Cavin1 shows strong localised clustering on the surface of Folch giant multilamellar vesicles (GMV) containing Bodipy-TMR-labelled PI(4,5)P2 (0.1 mol%). Scale bar = 5 μm Cavin1 (b), Cavin1-ΔDR3 (c) or Cavin1-ΔDR1 (d) were incubated with Folch GMVs containing Bodipy-TMR-labelled PI(4,5)P2 (0.1 mol%), allowed to settle on glass coverslips and images were acquired one frame per second. Frame numbers are indicated in PI(4,5)P2 channel (red). e GMVs incubated with Cavin1-ΔDR1 were often observed to be tethered to each other with Cavin1-ΔDR1 concentrated at the contact sites. Scale bar = 10 μm.

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