Fig. 8: Sequence requirements of the Cavin1 DR domains in caveola assembly. | Nature Communications

Fig. 8: Sequence requirements of the Cavin1 DR domains in caveola assembly.

From: Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation

Fig. 8

a Schematic diagram of Cavin1(1–345) with the sequences of the various point mutants indicated. DR1, DR2 and DR3 mutations occur in the regions 1–30, 161–197 and 311–345 respectively. b GFP-tagged Cavin1(1–345) DR1 domain mutants (green), c DR2 domain mutants and d DR3 domain mutants expressed in PC3 cells and immunolabelled with endogenous CAV1 (red). Images in b–d were by Airyscan confocal microscopy. Scale bar = 10 µm; enlarged boxes scale bar = 5 μm. e FRAP analysis of Cavin1 (1–345) DR3mut9 mutant showing fast recovery of fluorescence in cytosolic droplets and also droplet fusion events (marked by arrow). Scale bar = 10 µm; enlarged boxes scale bar = 5 μm.

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