Fig. 4: Destabilization of RNA helices by Zα correlates to base-pair stability.

a Regions identified as either being bound (green) or destabilized (red) by Zα (referring to Fig. 3) are overlayed onto the predicted base-pair free energy (RNAeval46). b Imino peak height for proton signals in AluSx1Jo, H66* (extended duplex), h43, and H25 RNAs which are destabilized due to Zα-binding are shown versus Zα concentration. c The inverse of the Zα concentration at which 90% of the imino signals disappear into the noise is shown. Peak heights in b are from 1D NMR titrations (one measurement for each RNA).