Fig. 1: 4T07 but not 4T1 breast cancer cells are dormant after dissemination to the lungs.
From: CD39+PD-1+CD8+ T cells mediate metastatic dormancy in breast cancer
a Diagram of the origin of 4T1 and 4T07 cell lines21. b Experimental design. 4T07-mCh cells (105) or PBS were injected into the mammary fat pad of female BALB/c mice. Analysis was performed 30 d later. c Quantification of disseminated 4T07-mCh cells by quantitative pathology, ****p < 0.0001. d 4T07-mCh cells (105) were injected into the mammary fat pad of female BALB/c mice. Analysis was performed 35 d later. Representative section of the primary tumor (upper panels) and lung (lower panels) at the endpoint. Disseminated 4T07-mCh cells were detected as single, Ki67-negative cells in the lungs of 5 out of 5 mice. Disseminated 4T07-mCh cells are shown in red, Ki67 in green, DAPI in blue. Scale bar indicates 50 µm. e Experimental design. 4T07-mCh cells (105) were injected into the mammary fat pad of female BALB/c mice. The tumor was resected 20 d after injection and analysis was performed 21 d after resection (endpoint). f Quantification of disseminated 4T07-mCh cells by quantitative pathology. ****p < 0.0001. g Representative section of the lung at the endpoint. Disseminated 4T07-mCh cells were detected as single, Ki67-negative cells in the lungs of 5 out of 5 mice. Disseminated 4T07-mCh cells are shown in red, Ki67 in green, DAPI in blue. Scale bar indicates 20 µm. Each symbol represents an individual mouse. Five mice per group. 2-tailed Student’s t-test. The bar represents the mean ± SD. Results are representative of three independent experiments.
