Fig. 3: Spermidine partially rescues impaired eIF5A function phenotypes in yeast. | Nature Communications

Fig. 3: Spermidine partially rescues impaired eIF5A function phenotypes in yeast.

From: Impaired eIF5A function causes a Mendelian disorder that is partially rescued in model systems by spermidine

Fig. 3

a Spermidine rescues growth of p.T48N and p.G106R strains. Growth of yeast strains for the human eIF5A (heIF5A) and the missense variants in minimum media supplemented with 0 (Untreated), 1 and 2 mM of spermidine at 30 °C. Eighteen replicates per temperature were performed. b Spermidine increases the interaction of heIF5A-T48N and heIF5A-G106R with ribosomes. Polysome profiles of heIF5A-WT, heIF5A-T48N and heIF5A-G106R grown at 30 °C in SC with 1 mM spermidine. Corresponding western blot analyses as described in the legend to Fig. 2c. Full uncropped images of gel blots are shown in Supplementary Fig. 12. One profile was performed. c Spermidine increases the synthesis of Ldb17 reporter in both p.T48N and p.G106R strains. Comparison of Ldb17 (left graph), total eIF5A (middle graph) and hypusinated eIF5A (right graph) among heIF5A yeast strains, grown at 30 °C in SCGal with and without 1 mM spermidine. Data presentation and statistics as described in the legend to Fig. 2b. Full uncropped images of gel blots are shown in Supplementary Fig. 13.

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