Fig. 3: A maximum likelihood tree of PAD sequences shows two clades of centiPAD sequences nested within a paraphyletic backbone of bacterial sequences.

The tree represents one clade nested within a larger tree (red highlight in inset) made up entirely of bacterial sequences. The tree shows that centiPADs originated from two bacterial HGTs, one within the lithobiomorph lineage, and one within the scutigeromorph lineage. Centipede sequences are in black (present in transcriptomes) and red (present in transcriptomes and venom proteomes). Highlighted sequences are Bacteria (pink) and Fungi (yellow). Metazoan sequences are not highlighted. Collapsed clades have the number of included sequences indicated in parentheses. For the uncollapsed tree see Supplementary Fig. 3. The tree was reconstructed using the WAG + G4 model and is displayed as midpoint rooted. Bootstrap support values are shown for each clade, and clades with support <50% are collapsed into polytomies. Clades without bootstrap values have >95% support. Collembolan image was sourced from Phylopic (www.phylopic.org; credit for the Collembola image is with Birgit Lang: https://creativecommons.org/licenses/by/3.0/).