Fig. 1: Perivascular and network niches in human and experimental gliomas.
From: Tumor cell plasticity, heterogeneity, and resistance in crucial microenvironmental niches in glioma
a Distribution of IDH1 R132H positive glioma cells and nestin positive glioblastoma cells in the perivascular and parenchymal compartment in molecularly defined patient glioma specimen (oligodendroglioma: n = 18 patients; astrocytoma: n = 19 patients; high grade astrocytoma: n = 20 patients; glioblastoma: n = 10 patients; one-way ANOVA, Tukey’s post hoc test). b Exemplary immunohistochemical stainings of IDH1 R132H in molecularly defined human glioma specimen and of nestin in human glioblastoma specimen demonstrate the dense interconnection of glioma cells in astrocytoma and glioblastoma specimen (left column) as well as the close association of tumor cells with blood vessels (arrowheads) (right column). c Exemplary two photon microscopy (2-PM) image of highly interconnected S24 glioblastoma stem-like cells (GBMSCs) growing in the mouse brain. d Intravital 2-PM image of perivascular S24 GBMSCs (arrowheads) in a mouse xenograft. e Exemplary immunofluorescence image of a human glioblastoma specimen (glioblastoma, IDH wild-type, ATRX retained, MGMT promoter unmethylated) demonstrates the dense interconnection of nestin-positive glioma cells and their relation to the perivascular niche (CD31-positive endothelium). Arrowheads exemplarily mark perivascular tumor cells. Data a are represented as violin plot with median and quartiles. (mut.: mutant; LOH: loss of heterozygosity; wt: wild-type). ***p < 0.001. Source data are provided as a Source Data file.
