Fig. 5: Perivascular cells govern glioma’s damage response.
From: Tumor cell plasticity, heterogeneity, and resistance in crucial microenvironmental niches in glioma
a Left: Early reaction (white arrowheads) of a perivascular cell (asterisk) after laser ablation of a nearby S24 GBMSC (pentagon). Cytoplasm (red), blood vessels (blue), nuclei (green). Right: Analysis of the early reaction to laser ablation of nearby cells (S24 glioblastoma stem-like cells (GBMSCs), n = 12 events, two-sided Fisher exact test). b After laser-induced stress (marked with gray area in the upper row) a strong perivascular reaction can be observed (S24 GBMSCs). c Representative in vivo 2-PM images of the same region before, 7 and 14 days after a surgical lesion (circle) in a S24 xenograft tumor. 7 days after lesioning there is a strong increase of the perivascular cell population (arrowheads). Upper row: overview; lower row: subset. Subset regions are marked by dotted squares. d Fold changes of perivascular (black) and parenchymal (red) cell counts 3 and 7 days after laser damage compared to before laser damage (S24 GBMSCs, n = 5 regions in 4 mice, two-tailed t-tests) indicate that the damage response is predominated by the PVN. e Quantification of the ratio of perivascular and parenchymal cells before, 7 and 14 days after a surgical lesion (S24 GBMSCs, n = 3 animals, one-way ANOVA (p = 3.92 × 10−5), Tukey’s post hoc test). This analysis reveals a shift towards the perivascular compartment that is most pronounced in the initial phase and precedes the repopulation of the lesioned brain area. Data d, e are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.
