Table 1 Interconnected tumor cells exhibit NOTCH1 pathway downregulation.

From: Tumor cell plasticity, heterogeneity, and resistance in crucial microenvironmental niches in glioma

Bulk RNA seq. of connected vs. non-connected tumor cells in vivo

NOTCH1 pathway activation

Gene

log2 fold change (S24 GBMSCs)

Adjusted p value (S24 GBMSCs)

log2 fold change (T269 GBMSCs)

Adjusted p value (T269 GBMSCs)

NOTCH1

−0.8411

0.0097

−0.6468

0.0122

HES6

−1.4296

6.2899e−6

−1.4490

5.4451e−8

DLL1

−2.3525

5.8021e−14

−2.6558

4.9849e−28

MFNG

−1.8398

1.018e−5

−2.8051

1.3372e−12

SATB1

−1.8029

1.8930e−6

−1.3847

1.9251e−8

MYCL

−1.2588

0.0016

−0.8474

0.0178

MYCN

−1.1416

0.0378

−1.0212

3.1898e−8

CDKN1C

−1.2697

0.0010

−2.3721

9.7218e−11

SOX8

−1.0591

1.3633e−4

−1.5615

4.3253e−27

ASCL1

−0.9464

0.0067

−1.1870

0.0081

PTPRJ

−0.8798

0.0350

−1.5500

0.0021

NOTCH1 pathway inhibition

Gene

 log2 fold change (S24 GBMSCs)

Adjusted p value (S24 GBMSCs)

log2 fold change (T269 GBMSCs)

Adjusted p value (T269 GBMSCs)

CDK1

 0.8195

0.0076

1.2082

7.9934e−5

CHI3L1

1.1131

3.7427e−4

1.5617

2.5644e−6

  1. Comparison of differential expression of genes associated with NOTCH1 pathway activation and inhibition respectively between connected and non-connected S24 and T269 orthotopically xenografted glioblastoma cells. Connected and nonconnected tumor cells were separated by FACS based on their uptake of SR101 in vivo before bulk RNA seq. was performed. Only genes with significant differential expression as determined by an adjusted p value < 0.05 are shown.
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