Fig. 5: SARS-2 S WT and D614G proteins are similarly neutralized by patient sera.
From: SARS-CoV-2 D614G spike mutation increases entry efficiency with enhanced ACE2-binding affinity
Lentiviruses pseudotyped with either the WT or D614G mutant SARS2-S were preincubated with two-fold serially diluted human sera (80-fold to 10, 240-fold) obtained from a healthy donor (Ctrl) or collected at 15–30 days post-symptom onset from confirmed case patients (#1–#5) infected with the prototypic viruses. The mixture was used for infection of 293T cells coexpressing ACE2 and TMPRSS2, and cell entry levels of pseudoviruses in the presence of diluted patient sera were determined by luciferase assays. Representative data from two independent experiments are shown as percent neutralization (mean ± s.d., n = 3 technical replicates). Source data are provided as a Source Data file.
