Fig. 3: The R615C mutation induces opening via disruption of Nsol–Bsol interactions.
From: Pathological conformations of disease mutant Ryanodine Receptors revealed by cryo-EM
a Superposition, based on the Nsol region, for WT (colors) and R615C (black) pig RyR1 (pRyR1). Alpha helices are represented as cylinders. This shows the movement of the Bsol relative to the Nsol, and the widening of the gap between the N-terminal domains of two neighboring subunits. The prime in NTD-A′ indicates that this is a different subunit. See also Movie S1. b Same view as in panel (a), showing the WT only, with yellow vectors showing the shifts >2 Å in the Bsol region compared to R615C pRyR1. c Side view of an overall superposition of WT (colors) and R615C (black) pRyR1. The major movements are indicated by arrows. Only two subunits are shown for clarity. See also Movie S2. d Same view as in panel (c), showing the WT pRyR1 only, with yellow vectors showing the shifts >2 Å throughout the protein going from WT to R615C pRyR1.
