Fig. 9: In the presence of apoCaM, the R615C mutation results in only subtle changes when comparing the open states of WT and mutant pRyR1.
From: Pathological conformations of disease mutant Ryanodine Receptors revealed by cryo-EM
a Superposition, based on the Nsol, of open WT pRyR1+apoCaM (colors) and open R615C pRyR1+apoCaM (black). In this state, the differences between WT and R615C are subtle. b Same view as in panel (a), but showing only the closed WT pRyR1 + apoCaM, with arrows indicating the extent of shifts >2 Å going from open WT pRyR1+apoCaM to open R615C pRyR1 + apoCaM. The extent of the shifts is much smaller in relation to the closed structures (Fig. 8b). c Overall superposition of open WT pRyR1+apoCaM (colors) and open R615C pRyR1+apoCaM (black), with a close-up around the NTDs of different subunits, highlights the similarity between WT and R615C in this state. d Superposition, based on the Nsol, of all six structures described in this study. This highlights the range in the angle of the Bsol relative to the Nsol, in the order WT (no CaM) ≈ closed WT + apoCaM < closed R615C + apoCaM < open WT + apoCaM < open R615C + apoCaM ≈ R615C (no CaM).
