Fig. 3: Solid-state NMR of DnaJB8 oligomers at physiological and high ionic strength.
From: Regulatory inter-domain interactions influence Hsp70 recruitment to the DnaJB8 chaperone
a 2D 13C-13C ssNMR spectrum of U-13C,15N-labeled DnaJB8 oligomers in PBS (100 mM NaCl), using 25 ms DARR mixing. b Corresponding 2D ssNMR spectrum in PBS with 285 mM NaCl. c, d Boxed Ala and Ser regions from panels (a and b). In PBS, the experimental Ala and Ser peak patterns (black) are well resolved and similar to those expected for folded JD in solution (red). At elevated ionic strength (brown) these narrow peaks are missing. Green spectra (right) represent simulated signals predicted for our models of the non-JD domains, shown with enhanced broadening reflecting the heterogeneity seen in the experiments. 1D spectra on far left show slices through the experimental 2D data, with selected peak widths (in kHz). e 13C 1D spectra, in PBS (top) and with 285 mM NaCl (bottom), that show rigid residues (black, CP), rigid and mobile residues (green, SPE), and only mobile residues (red, INEPT). See also text and Supplementary Fig. 3.
