Fig. 2: Structure of Arabidopsis ATC bound to UMP. | Nature Communications

Fig. 2: Structure of Arabidopsis ATC bound to UMP.

From: Mechanisms of feedback inhibition and sequential firing of active sites in plant aspartate transcarbamoylase

Fig. 2

a Scheme of Arabidopsis ATC protein. b SEC-MALS analysis of purified atATC proves the formation of a homotrimer. c Immunoblot of atATC and mature ATC from leaf extract. The experiment was performed three times with identical results. d Crystal structure of atATC trimer with each subunit bound to one molecule of UMP (shown as yellow spheres). One subunit is shown on gray background. Protein domains are colored as in (a). e Detail of the active site with UMP bound. Water molecules are shown as red spheres. Electrostatic interactions are indicated as dashed lines.

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