Fig. 1: Response to CAR T cell treatment and microenvironment changes. | Nature Communications

Fig. 1: Response to CAR T cell treatment and microenvironment changes.

From: Biallelic loss of BCMA as a resistance mechanism to CAR T cell therapy in a patient with multiple myeloma

Fig. 1

A M spike and lambda free light chain evaluations for the patient. The y axis on left shows the M spike values (blue) and on the right it shows the lambda free light chain values (green). Time points (x axis) marked with red labels also shows the longitudinal sample collection for single-cell RNA sequencing. B Expansion of CAR T cells (y axis) measured with qPCR after first (blue) and second (red) infusions from day 0 to day 60 (x axis). C Timeline of the eight samples collected for single-cell RNA sequencing. D Thirteen single-cell clusters from eight longitudinal bone marrow samples. Annotation of cell clusters are marked in the bottom part with color codes. Cell embedings are shown by using UMAP1 and UMAP2. E Ide-cel expression in single cells. Only limited number of cells are CAR+ at 2 weeks after the first infusion. None of the other time points show CAR+ cells. F Re-clustered T cells divided by time points from study screening to 1 month after second infusion and T-cell annotations for CD4+ and CD8+ cells are shown with color codes. G Percentage of particular T-cell types (y axis) at each time point (x axis) evaluated with single-cell RNA seqeuecning for T-cell clusters (top figure legend). Percentages are reflecting the % of particular cluster at given time point within all T cell populations. H Gene-set enrichment FDR values for differentially expressed genes for two samples collected two weeks after first (blue) and second (green) infusions. I Percentage of T cells (y axis) expressing immune checkpoint inhibitors at each time point (x axis).

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