Fig. 1: Reentrant phase separation of FUS at high salt.

Phase diagram (left), representative images (center), and schematic (right) of FUS phase separation in the presence of increasing concentrations of KCl. In the phase diagram, markers filled with blue indicate concentrations where phase separation was observed in fluorescence images. Open markers indicate concentrations tested where phase separation did not occur. Darker blue regions are guides for the eyes indicating regions where phase separation of FUS occurs, and light blue is the region where no phase separation occurs. The reentrant phase separation regime is indicated. Fluorescent images of FUS (6 μM, EGFP labeled) were taken at 50 mM (low salt), 500 mM (intermediate salt), and 2.7 M KCl (high salt) in 50 mM Tris-HCl (pH 7.2). Scale bar is 20 µm. Each data image (center panel) is representative of the observed behavior from at least three test replicates of the respective protein/salt conditions.