Fig. 5: Hofmeister effect in the high-salt phase separation behavior of FUS and PR25.
a Phase diagram for FUS as a function of salt concentration of various salts of the Hofmeister series. Open circles indicate cases where phase separation did not occur, closed circles indicate where phase separation did occur. Each curve depicts the apparent phase boundary for the particular salt named next to it and is only meant as a guide for the eyes. Even at the saturation concentration of CaCl2 (gray), the hydrophobic effect is weakened to the extent such that phase separation cannot occur, indicated by the presence of open circles and absence of closed ones in the phase diagram. b Phase behavior of PR25 as a function of ionic strength of various salts. The trend is consistent with panel a. c Comparison of the amount of 1,6-hexanediol required to dissolve FUS condensates in solutions of various salts along with the Hofmeister series. In each solution, the final salt concentration was 4 M, and the final FUS concentration was 2 μM. Partially shaded circles represent conditions where the number of condensates was visibly reduced, but the condensates were not fully dissolved. d Comparison of the amount of 1,6-hexanediol required to dissolve PR25 as a function of salts along the Hofmeister series. The final salt concentration at each point was 4 M and the PR25 concentration was 100 μM.
