Fig. 4: dCas9-dMSK1 induces target gene activation via H3S28ph. | Nature Communications

Fig. 4: dCas9-dMSK1 induces target gene activation via H3S28ph.

From: Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase

Fig. 4

a, b ChIP-qPCR enrichment for H3S10ph and H3S28ph, at the OCT4 (panel a) or MYOD (panel b) promoters 72 h post-transfection of dCas9 or indicated dCas9-fusion protein and corresponding gRNAs. c, d Re-ChIP-qPCR enrichment for H3S10ph and H3S28ph at OCT4 (panel c) or MYOD (panel d) promoters 72 h post-transfection of dCas9 or indicated dCas9-fusion protein and corresponding gRNAs. Two-sided t-test, *P < 0.05; n = 3 independent experiments for all panels; error bars, s.e.m.; ns, not significant. Source data are available in the Source data file.

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