Fig. 6: m6A is a driving force in L1 evolution.
From: L1 retrotransposons exploit RNA m6A modification as an evolutionary driving force
a Comparative analysis of L1 A332 m6A sites in species-specific full-length L1s from three primates. Phylogenetic tree of gorilla, chimpanzee, and human L1s with predicted age and the corresponding L1 subfamily lineages (left). Changes in the A332-m6A motif region from L1PA3 or older L1s to L1PA2 and a younger L1 (right). The substitution site wherein the residue converts from T to C (333) is highlighted in yellow. The percentage indicates the proportion of m6A motif-positive L1s with nucleotide C to total L1s. b A schematic of retrotransposition assay using pL1PA25′ UTR construct that is generated by substituting 5′ UTR of pL1Hs with A332 m6A negative 5′ UTR of L1PA2. A schematic of T333C m6A acquisition mutagenesis in L1 5′ UTR 328–336 region was indicated in red. c Retrotransposition assays for assessing the effect of A332 m6A acquisition in pL1PA25′ UTR with T333C mutation. T333G mutation served as negative control (n = 3 independent samples, mean ± s.d., one-way ANOVA and Tukey’s multiple comparison test; **p < 0.01). d Immunoblot assay showing L1 ORF1p expression in the indicated pL1-transfected HeLa cells. HSP70 served as a loading control. The immunoblot images are representative of three independent experiments. Source data are provided as a Source data file.
