Fig. 3: RANTES synthesis. | Nature Communications

Fig. 3: RANTES synthesis.

From: General synthetic strategy for regioselective ultrafast formation of disulfide bonds in peptides and proteins

Fig. 3

a HPLC-ESI MS analyses: Reaction at time zero, the main peak corresponds to reduced RANTES modified with two Acm groups and two free Cys with the observed mass 7991.3 ± 0.1 Da, calcd. 7992.0 Da (average isotopes). b Reaction after 1 min: the main peak corresponds to RANTES bearing one disulfide bond and modified with two Acm with the observed mass 7989.6 ± 0.3 Da, calcd. 7990.0 Da (average isotopes). c Reaction after 5 min:HPLC-ESI MS, the main peak corresponds to RANTES bearing two disulfide bonds with the observed mass 7844.6 ± 0.1 Da, calcd. 7846.0 Da (average isotopes). d Purification and folding: the main peak corresponds to RANTES bearing two disulfide bonds with the observed mass 7844.7 ± 0.1 Da, calcd. 7846.0 Da (average isotopes). e commercially available native RANTES: analyses with the observed mass 7843.9 ± 0.1 Da, calcd. 7846.0 Da (average isotopes). f CD spectrum of the synthetic RANTES.

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