Fig. 4: Synthesis of plectasin employing our synthetic design. | Nature Communications

Fig. 4: Synthesis of plectasin employing our synthetic design.

From: General synthetic strategy for regioselective ultrafast formation of disulfide bonds in peptides and proteins

Fig. 4

a HPLC-ESI MS analyses Reaction at time zero, the main peak corresponds to reduced plectasin with the observed mass 4801.1 ± 0.1 Da, calcd. 4801.0 Da (average isotopes). b Reaction after 10 s: the main peak corresponds to plectasin with single disulfide bond, with the observed mass 4798.2 ± 0.1 Da, calcd. 4798.0 Da (average isotopes). c Reaction after 8 min, the main peak corresponds to plectasin with two disulfide bonds with the observed mass 4526.2 ± 0.2 Da, calcd. 4527.0 Da (average isotopes). d Reaction after 13 min, the main peak corresponds to fully oxidized plectasin with the observed mass 4382.9 ± 0.1 Da, calcd. 4383.0 Da (average isotopes). e Plectasin activity assay: absorbance monitoring at 600 nm for methicillin resistant staphylococcus aureus (MRSA) growth after 7 h in the absence (in light blue) and presence (in light red) of synthetic plectasin and MRSA in the presence of trimethoprim (TMP) (in gray). LB was used as medium in the assay (in dark red). Data are represented as mean ± SD, n = 3 biologically independent samples, error bars represent the SD. IC50 ~1.5–2 µM. R1 = NBzl, R2 = Acm. f Plectasin CD.

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