Fig. 5: Linaclotide synthesis. | Nature Communications

Fig. 5: Linaclotide synthesis.

From: General synthetic strategy for regioselective ultrafast formation of disulfide bonds in peptides and proteins

Fig. 5

a HPLC-ESI MS analyses: Reaction at time zero, the main peak corresponds to reduced Linaclotide with the observed mass 1943.1 ± 0.1 Da, calcd. 1944.2 Da (average isotopes). b Reaction after 10 s, the main peak corresponds to Linaclotide with single disulfide bond with the observed mass 1941.7. ± 0.1 Da, calcd. 1941.1 Da (average isotopes). c Reaction after 8 min: the main peak corresponds to Linaclotide with two disulfide bonds with the observed mass 1668.5 ± 0.2 Da, calcd. 1669.1 Da (average isotopes). d Reaction after 5 min: the main peak corresponds to Linaclotide with the three disulfide bonds with the observed mass 1525.1 ± 0.1 Da, calcd. 1525.1 Da (average isotopes). R1 = NBzl, R2 = Acm. *non peptide mass correspondence to small molecule decomposition.

Back to article page