Fig. 6: EETI-II synthesis. | Nature Communications

Fig. 6: EETI-II synthesis.

From: General synthetic strategy for regioselective ultrafast formation of disulfide bonds in peptides and proteins

Fig. 6

a HPLC-ESI MS analyses: Reaction at time zero, the main peak corresponds to reduced EETI-II with the observed mass 3297.6 ± 0.1 Da, calcd. 3297.4 Da (average isotopes). b Reaction after 10 s, the main peak corresponds to EETI-II with single disulfide bond with the observed mass 3295.2 ± 0.1 Da, calcd. 3295.4 Da (average isotopes). c Reaction after 8 min: the main peak corresponds to EETI-II with two disulfide bonds with the observed mass 3022.5 ± 0.2 Da, calcd. 3023.4 Da (average isotopes). d Reaction after 13 min: the main peak corresponds to EETI-II with three disulfide bonds with the observed mass 2879.2 ± 0.1 Da, calcd. 2879.4 Da (average isotopes). R1 = NBzl, R2 = Acm.

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