Fig. 5: HMGA2 -FACT interaction is required for pH2A.X deposition. | Nature Communications

Fig. 5: HMGA2 -FACT interaction is required for pH2A.X deposition.

From: Positioning of nucleosomes containing γ-H2AX precedes active DNA demethylation and transcription initiation

Fig. 5

a Western blot using the indicated antibodies after co-immunoprecipitation (Co-IP) assay using nuclear protein extracts from Hmga2−/− MEF that were non-transfected (−) or stably transfected with Hmga2-myc-his (WT) and magnetic beads coated with MYC-specific antibodies. Representative images from three independent experiments. Input, 5% of IP starting material. b, c ChIP-based promoter analysis of selected HMGA2 target genes using the indicated antibodies and chromatin from Hmga2 + /+, Hmga2−/− MEF, as well as Hmga2−/− MEF that were stably transfected with a tetracycline-inducible expression construct (tetOn) for WT Hmga2-myc-his. MEF were treated with doxycycline and FACT inhibitor (FACTin; CBLC000 trifluoroacetate) as indicated. In all bar plots, data are shown as means ± s.e.m. (n = 3 biologically independent experiments); asterisks, P-values after one-tailed t-Test, ***P ≤ 0.001; **P ≤ 0.01; *P ≤ 0.05; ns, non-significant. See also Supplementary Fig. 5. Source data are provided as a Source Data files 01 and 02.

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