Fig. 4: Mass cytometric single cell analysis identifies phenotypic cell clusters that differ significantly between primitive vs. committed NPM1-mutated AML cases.

A tSNE plots of FlowSom immunophenotypic clusters in representative primitive and committed cases with the indicated FLT3-ITD genotypes. Twenty-five unique FlowSom immunophenotypic clusters were identified and are colored in the Z dimension according to the color scale on the right. The numerical IDs of key leukemic (black numbers) and normal non-leukemic (red numbers) immunophenotypic clusters are labeled. B Stacked bar-plot of FlowSom immunophenotypic cluster abundance (% of total) in each sample grouped according to FLT3-ITD status within the primitive vs committed groups. immunophenotypic clusters identified by diffcyt as differentially abundant between the two groups (FDR(Q) < 0.05) are boxed as “Significant” in the legend. Leukemic versus normal non-leukemic immunophenotypic clusters were identified based on marker expression (Supplementary Figs. 20, 21). To simplify visualization, very low abundance (<5% of total cells) non-significant leukemic (gray) and all normal non-leukemic immunophenotypic clusters (black) were aggregated. C Heatmap representation of the median metal intensity of each marker for each differentially abundant immunophenotypic cluster, represented as the Arcsinh ratio. D Box and whisker plots of leukemic immunophenotypic clusters that were differentially abundant between the primitive and committed groups in the diffcyt analysis (n = 9 in primitive and n = 8 in committed group). The whiskers represent the 1.5 × interquartile range (IQR) extending from the hinges. Q-values for each from the diffcyt-DA-edgeR analysis are indicated.